基于多指标含量测定结合化学计量学的不同产地丹参品质差异分析

Quality Difference Analysis of Salviae Miltiorrhizae Radix et Rhizoma from Different Origins Based on Multi-index Content Determination Combined with Chemometrics

中文关键词:丹参  产地差异  含量测定  化学计量学  质量评价

Key Words:Salviae Miltiorrhizae Radix et Rhizoma  origin difference  content determination  chemometrics  quality evaluation

中文摘要:为对比分析产地对丹参中酚酸类和丹参酮类成分的影响，采用超高效液相色谱（UPLC）及超高效液相色谱－三重四极杆质谱联用技术（UPLC－QQQ－MS）同时测定来自山东、河南、陕西、四川、安徽共408份丹参中23种化学成分的含量，并对数据进行多元统计分析。研究发现17种酚酸类及6种丹参酮类成分在不同产地丹参中均存在显著差异。山东的丹参样品中丹参酮类成分含量最高，四川的样品中丹酚酸B含量最高，安徽丹参的紫草酸、丹酚酸Y、丹酚酸A、丹酚酸D和丹酚酸E等的含量最高。多种模式识别方法均可用于不同产地丹参的判别分析，线性判别分析（LDA）为产地溯源的最佳模型。正交偏最小二乘法判别分析（OPLS－DA）表明不同产地丹参的化学成分差异较大，不同来源丹参的质量差异标志物不仅限于丹酚酸B、丹参酮Ⅰ、隐丹参酮、丹参酮ⅡA，其他丹酚酸类及丹参酮类也是重要的质量标志物。该研究对全国不同主产区的栽培丹参进行多指标含量测定及建模分析，所建立的定量方法专属性强、准确高效，可为不同产地丹参的质量控制及产地判别提供参考。

Abstract:Ultra-performance liquid chromatography（UPLC） and ultra-performance liquid chromatography－triple quadrupole mass spectrometry（UPLC－QQQ－MS） were adopted for the simultaneous determination on 23 chemical ingredients in a total of 408 Salviae Miltiorrhizae Radix et Rhizoma（Danshen） samples from Shandong，Henan，Shaaxi，Sichuan and Anhui provinces，in order to compare and analyze the influences of origins on phenolic acids and tanshinones in Danshen．Meanwhile，the data were analyzed by multivariate statistics．The results showed that 17 phenolic acids and 6 tanshinones had significant differences in Danshen．The samples from Shandong province had the highest content of tanshinones，while the samples from Sichuan province had the highest content of salvianolic acid B，and the samples from Anhui province had the highest contents of lithospermic acid，salvianolic acid Y，salvianolic acid A，salvianolic acid D and salvianolic acid E．Various pattern recognition methods were used to identify and analyze Danshen from different origins，and linear discriminant analysis（LDA） was the best model for traceability．Orthogonal partial least squares－discriminant analysis（OPLS－DA） showed that the chemical constituents of Danshen from different origin areas were significantly different．Quality markers of Danshen from different sources were not limited to salvianolic acid B，tanshinone Ⅰ，cryptotanshinone and tanshinone ⅡA，other salvianolic acids and tanshinones were also important quality markers. In this paper，the multi-component content determination and data analysis of cultivated medicinal materials collected from different main origin areas of the country were carried out．The quantitative method established was highly specific，accurate and efficient，which provided a reference for the quality control and identification of Danshen from different producing areas.