高效液相色谱法同时测定化妆品中辣椒碱等9种热感剂 |
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中文摘要:建立了测定化妆品中辣椒碱等9种热感剂的高效液相色谱法。样品经80%甲醇超声提取后,过滤,以乙腈-0.5%甲酸水溶液为流动相,采用SymmetryShieldTMRP18色谱柱(250 mm × 4.6 mm,5 μm)分离,液相色谱分离,二极管阵列检测器进行检测。在优化实验条件下,9种热感剂的分离度好,专属性高,其质量浓度在0.2 ~ 100 μg/mL范围内与峰面积呈良好线性关系,相关系数(r2)均不小于0.999 5,检出限(LOD)为0.001 0 ~ 0.003 4 mg/kg。以水基类、乳剂类、膏霜类3种阴性样品作为空白基质,9种热感剂在0.004、0.02、1 mg/kg 3种加标水平下的回收率为75.6% ~ 115%,相对标准偏差(RSD)为0.083% ~ 7.0%。该方法专属性强、灵敏度高、准确度好,可用于化妆品中9种热感剂含量的测定。 |
中文关键词:热感剂 化妆品 高效液相色谱法(HPLC) |
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Simultaneous Determination of 9 Self-heating Compounds Including Capsaicin in Cosmetics by High Performance Liquid Chromatography |
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Abstract:A high performance liquid chromatographic(HPLC) method was established for the determination of 9 self-heating compounds,including capsaicin in cosmetics.The samples were ultrasonically extracted with 80% methanol,and then filtered.The target compounds were separated on a SymmetryShieldTMRP18(250 mm × 4.6 mm,5 μm) with a mobile phase consisting of acetonitrile and 0.5% formic acid by gradient elution,and analyzed by HPLC with a PDA detector.Under the optimal conditions,good linear relationships were obtained for nine compounds in the concentration range of 0.2-100 μg/mL,with correlation coefficients(r2) not less than 0.999 5.The limits of detection(LODs) were in the range of 0.001 0-0.003 4 mg/kg.The spiked recoveries for water,lotion and cream matrixes at three levels of 0.004,0.02,1 mg/kg ranged from 75.6% to 115%,with relative standard deviations(RSDs,n = 3) of 0.083%-7.0%.This method is accurate and sensitive,and is suitable for the qualitative and quantitative analysis of 9 self-heating compounds in cosmetics. |
Key Words:self-heating compounds cosmetics high performance liquid chromatography(HPLC) |
引用本文:吴志珊,吴姣娇,尹伟成,殷帅,李卓芳,姜成君.高效液相色谱法同时测定化妆品中辣椒碱等9种热感剂[J].分析测试学报,2023,(5):641-646. |
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