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Determination of 6 Plant Growth Retardants in Fruits and Vegetables by HPLC-MS/MS |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:Key words:high liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI-MS/MS) plant growth retardants(PGRs) residues |
Author | Institution |
WANG Jingjing1,LU Yi2,YANG Tao2,ZHANG Huangtao2,WANG Jide1 |
1.石油天然气精细化工教育部自治区重点实验室;2.新疆产品质量监督检验研究院 |
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Abstract: |
A comprehensive analytical method based on high performance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI MS/MS) under positive ionization mode was developed for the determination of 6 plant growth retardants(PGRs) residues,including choline chloride,chlormequat chloride,mepiquat chloride,ancymidol,paclobutrazol and uniconazole in fruits and vegetables.The samples were extracted with methanol by ultrasonic wave assistant extraction(UAE).The extract was purified and concentrated by solid phase extraction(SPE) with Waters Oasis HLB column(3 mL,60 mg).The separation of the target compound was performed with Thermo Hypersil Gold aQ(21 mm×150 mm,3 μm) column by gradient elution.The identification of 6 PGRs was carried out by ESI under the positive ion mode.Effects of mobile phase and additives on ionization were investigated.Effects of the types and amounts of extraction solvents and the types of SPE column were also studied.Under the optimal conditions,the calibration curves of 6 PGRs were linear in the range of 10-2000 μg/L with correlation coefficients(r) more than 099.Recoveries of 6 PGRs at three spiked concentration levels of 20,100,500 μg/kg ranged from 81% to 104% with relative standard deviations no more than 50%.The detection limits(LOD) were in the range of 015-035 μg/kg and the quantitative limits(LOQ) were 047-110 μg/kg.The established method has the advantages of high sensitivity,nice selectivity and low detection limit,and was applied in the determination of PGRs in fruits and vegetables with satisfactory results. |
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