Separation of Polyacrylic Acid and Determination of Its Relative Molecular Mass by Gel Permeation Chromatography

KeyWord:polyacrylic acid  molecular weight  gel permeation chromatography(GPC)  non size exclusion effect

Abstract:

      Using Sepax Nanofilm SEC 150 chromatographic column and self made narrow distribution polyacrylic acid£¨PAA£© standard sample£¬effects of different inorganic salts and their ionic strength as well as pH value on the separation of PAA were investigated.The results indicated that the different relative molecular masses of PAA with mass concentration of 0ª±5-2ª±0 g/L could be separated by using ionic strength of 0ª±10 mol/L and pH 6ª±9 phosphorous buffer as mobile phase£¬with a flow rate of 1ª±0 mL/min£¬column temperature of 25 ¡æ and sample volume of 50 ¦ÌL.Under the optimal chromatographic conditions£¬the relative molecular mass and its distribution of PAA sample were measured accurately by the asymptotic calibration method£¬and the difference between result of viscometric and GPC methods was less than 10%.