Determination of Buddleoside by Capillary Electrophoresis

KeyWord:capillary electrophoresis  buddleoside  amperometric detection

Abstract:

      Using self constructed electrochemical detection system£¬buddleoside was detected by high performance capillary electrophoresis(HPCE).Several parameters affecting the determination were studied£¬including effects of potential of working electrode£¬organic concentration,running buffer£¬acidity and working voltage.Under the optimal conditions£¬a linear relationship between the peak height£¨Y£© and the mass concentration£¨c£© was obtained in the range of 2ª±0-80 mg/L with a correlation coefficient of 0ª±999 6.The determination limit was 0ª±2 mg/L and the average recovery was 99%.