A capillary zone electrophoresis(CZE) method was developed for the separation and quantitative analysis of water buffalo caseins.The effects of several parameters,including buffer system and concentration,pH value,urea concentration and injection time on the separation efficiency of water buffalo caseins were investigated on an uncoated fused silica capillary column(50.2 cm×75 μm id) using separation voltage of 25 kV and detection wavelength of 214 nm at 25 ℃.Under the optimal conditions,the calibration curves were linear in the range of 0.5-5.0 g/L for α CN,β CN and κ CN with correlation coefficients no less than 0.998 0.The spiked recoveries of three main caseins were in the range of 98%-106%.The relative standard deviations(RSDs) for migration time,peak area and peak height were no more than 067%,3.0% and 3.2%,respectively.This method was simple,rapid and accurate,and could be used for the quality control and quantitative analysis of water buffalo milk and dairy products. |