Study on Determination of Glucoside by Gas Chromatography with Electron Capture Detector

KeyWord:glucoside  GC/ECD  N methyl bis£¨trifluoroacetamide£©  derivatization  determination

Abstract:

      A GC/ECD method was developed for the determination of glucosides.The target compound£¨phenyl ¦Â D glucopyranoside£© was derivatized with N methyl bis£¨trifluoroacetamide£©£¨MBTFA£©.The derivatization temperature and time were optimized.The result indicated that the best derivatization conditions were achieved at 60 ¡æ for 50 min.Fluorine was introduced to the product molecule after derivatization to provide the fundamental element for the detection of GC/ECD.Linear study was conducted by using phenyl ¦Â D glucopyranoside as a typical compound,and a correlation coefficient£¨r2£© of 0.999 6 was obtained in the range of 0.05-200 mg?L-1.Six glucosides could be accurately identified by GC/ECD after MBTFA derivatization.Repeatability experiment showed that this derivatization combined with GC/ECD determination had a good performance,the relative standard deviation for the corresponding peak area was lower than 3% in 72 h.This method was also compared with the GC-MS method,and the limit of detection of GC/ECD method was greatly lowered.The proposed method is applicable in the determination of trace amount of glucosides in real sample.