Determination of Aliphatic Aldehydes in Wine by High Performance Liquid Chromatography with BODIPY based Hydrazine Reagent

KeyWord:fluorescent labeling reagent  1,3,5,7.tetramethyl.8.propionohydrazide difluoroboradiaza s indacene  aliphatic aldehydes  high performance liquid chromatography  wine

Abstract:

      A new fluorescent labeling reagent,1,3,5,7-tetramethyl-8-propionohydrazide difluoroboradiaza s indacene (BODIPY propionohydrazide£©,was synthesized for the determination of C1-C12 aliphatic aldehydes by high performance liquid chromatography(HPLC£©. The experimental conditions were optimized. In the presence of phosphorous acid as a catalyst£¬the derivatization reaction of BODIPY propionohydrazide with aldehydes was completed in 30 min at 60 ¡æ.The stable BODIPY hydrazone derivatives(except for formaldehyde and acetaldehyde derivatives£© could be separated in 20 min with acetonitrile£¨containing 20% tetrahydrofuran£© and HCOOH-NH3?H2O as mobile phase£¨pH=6.5£©. Under the optimal conditions,the calibration curves of C1-C12 aliphatic aldehydes were linear in the range of 0.01-2.00 ¦Ìmol?L-1 with detection limits(S/N=3£© of 0.23-0.78 nmol?L-1. The established method was applied in the determination of aliphatic aldehydes in wine samples with spiked recoveries of 95%-105%.