Study on the Volatile Constituents of Acorus tatarinowii Schott from Different Growing Areas

KeyWord:Acorus tatarinowii Schott  gas chromatography-mass spectrometry  volatile constituents  dividing sections

Abstract:

      The dry rhizome of Acorus tatarinowii Schott is a common traditional Chinese medicine and could be used for treatment of lots of diseases,such as epilepsy.In this paper,the volatile constituents of Aª±tatarinowii Schott from 4 growing areas,eª±gª±Anqing,Anhui;Jinhua,Zhejiang;Songxian,Henan and Zhumadian,Henan,were investigated by gas chromatography-mass spectrometry(GC-MS).Total of 45 volatile constituents were tentatively identified and their relative contents were determined.There were 4 common volatile constituents(¦Â asarone,¦Ã asarone,¦Á asarone and cis methylisoeugenol) in Aª±tatarinowii Schott,which occupied more than 1% of the contents of the volatile constituents.Their retention time and relative contents could be regarded as the essential condition of the identification and quality assessment on Aª±tatarinowii Schott.In application of the dividing sections,the total ion current chromatograms of Aª±tatarinowii Schott were divided into 4 sections and were further compared with the relative retention value(¦Á) and the relative peak area(Sr).It was shown that the 3rd section of the total ion current chromatograms was the major active ingredient of Aª±tatarinowii Schott and consisted of the 28th,30th and 36th peaks which corresponded to ¦Ã asarone,¦Â asarone and ¦Á asarone,respectively.This study provided a new approach for the comparison and quality assessment of Aª±tatarinowii Schott from different growing areas.