Determination of Triazole Ultraviolet Stabilizer in Composite Laminated Food Packaging Bags by High Performance Liquid Chromatography

KeyWord:UV 326  UV 327  ultraviolet stabilizer  composite laminated food packaging bags  high performance liquid chromatography£¨HPLC£©

Abstract:

      A new high performance liquid chromatographic£¨HPLC£© method with ultraviolet detection was firstly developed for the determination of 2 £¨3 tert butyl 2 hydroxy 5 methylphenyl£© 5 chloro 2h benzotriazole£¨UV 326£© and 2 £¨2 hydroxy 3,5 di tert butylphenyl£© 5 chlorobenzotriazole£¨UV 327£© in composite laminated food packaging bags.The samples were extracted by ultrasound assisted extraction method.Then£¬the extracts were further concentrated by rotary evaporation technology and cleaned up with solid phase extraction column.The eluent was evaporated to near dryness under a stream of nitrogen and redissolved with methanol.The separation of target compounds was performed with Agilent ZORBZX SB C18 column£¨250 mm¡Á4.6 mm,5 ¦Ìm£© using methanol as elution reagent at a flow rate of 1.0 mL/min at 30 ¡æ.The quantitative analysis of target compounds was performed by the external standard method at a detection wavelength of 310 nm.The result indicated that the calibration curves were linear in the range of 0.2-10 mg/kg for UV 326 and UV 327 with both limits of quantitation of 0.2 mg/kg.The average recoveries of UV 326 and UV 327 were in the range of 75%-90% and 80%-85%,respectively£¬with RSDs of 3.6%-6.7% and 3.4%-4.2%,respectively.The method could be applied in the determination of UV 326 and UV 327 in composite laminated food packaging bags.