Determination of Amantadine Hydrochloride in Amantadine Hydrochloride Tablets by Microfluidic Chip Method

KeyWord:microfluidic chip  contactless conductivity detection  amantadine hydrochloride  determination

Abstract:

      A new method of microfluidic chip with contactless conductivity detection was established for the determination of amantadine hydrochloride in amantadine hydrochloride tablets£®The electrophoretic parameters£¬such as the variety and concentration of buffer solution and additive£¬separation voltage and injection time etc£¬were investigated£®Under the optimal conditions£¬using 1 mmol/L HAc+2 mmol/L NaAc£¨pH 4.5£©+0.1 mmol/L SDS as buffer solution system£¬amantadine hydrochloride was separated and detected in 1 min at the separation voltage of 2.00 kV and 10 s injection time£®The linear dependence of the concentration of amantadine hydrochloride ranged from 10 mg/L to 120 mg/L £¨r=0.999 0£©£¬with a detection limit£¨S/N=3£© of 0.6 mg/L.The spiked recoveries ranged from 97% to 98% with RSDs of 0.8%-2.2%.The method was simple£¬rapid and well reproducible£¬and could be used for the rapid detection and the quality control of the product.