Determination of Four Trace Phthalates in Water by Online SPE Coupled with High Performance Liquid Chromatography

KeyWord:phthalate  online solid phase extraction  high performance liquid chromatography  water sample

Abstract:

      A method for the simultaneous determination of four trace phthalates£¨PAEs£©£¬eª±gª±dimethyl phthalate,diethyl phthalate,dibutyl phthalate and bis(2 ethylhexyl) phthalate,in water samples was established using high performance liquid chromatography with the function of online column enrichment.After injected with an assistant pump and concentrated at the solid phase extraction cartridge,the PAEs were back flushed to the analytical column by a six port valve switching for detection with mobile phase.The online SPE cartridge was IonPac¡k NG1(10 ¦Ìm¡Á4.0 mm¡Á35 mm) and the LC column was Acclaim¡k 120¦@ C18(5 ¦Ìm¡Á4.6 mm¡Á150 mm).The quantitation analysis of four phthalates were achieved by UV detection at 224 nm with 15 mL sample load volume.The linear ranges of four PAEs were in the range of 1-100 ¦Ìg/L with correlation coefficients(r2) not less than 0.999 8.The method detection limits(S/N=3) of four PAEs were between 0.10 ¦Ìg/L and 0.25 ¦Ìg/L.The relative standard deviations(RSDs) of five injections of 10 ¦Ìg/L working solution were less than 2%.The spiked recoveries at two matrix spiked concentrations were in the range of 88%-107%.The results showed that the developed method was sensitive and accurate,and was suitable for the analysis of trace PAEs in water sample.