Simultaneous Determination of Fat soluble Vitamins A,E and K3 in Dried Okra by High Performance Liquid Chromatography

KeyWord:high performance liquid chromatography£¨HPLC£©  fat-soluble vitamins  vitamins A,E,K3  okra

Abstract:

      A method was developed for the simultaneous determination of fat-soluble vitamin A,E and K3 in dried okra by reversed phase high performance liquid chromatography coupled with diode array detection.The sample was first extracted with ethanol and followed by saponification with 50% KOH solution,then extracted with petroleum ether.The extract was separated on an Agilent Eclipse XDB C18 column£¨150 mm¡Á 4.6 mm,5 ¦Ìm i.d.£© at 30 ¡æ,using methanol as mobile phase at a flow rate of 1.0 mL/min.The target compounds were simultaneously detected at 325,290,244 nm,respectively,and quantified by the external standard method.The results showed that the linear ranges for fat-soluble vitamins A,E,K3 were in the range of 0.1-20.0 mg/L with correlation coefficients more than 0.99.The spiked recoveries of three fat-soluble vitamins from dry okra sample were in the range of 88%-95% with RSDs of 0.8%-3.9%.The limits of detection£¨LODs,S/N=3£©of VA,VE and VK3 were 0.3,0.6,0.1 mg/kg,respectively,and the limits of quantitation£¨LOQs,S/N=10£© were 1.0£¬2.0,0.3 mg/kg,respectively.The developed method was simple and accurate,and could meet the demands for actual detection.