Synthesis of Ceftiofur Artificial Bovine Serum Albumin Antigen and Its Identification by Atomic Force Microscope

KeyWord:ceftiofur  artificial antigen  atomic force microscope(AFM)

Abstract:

      The ceftiofur artificial antigen was synthesized by conjugating ceftiofur to BSA with n hydroxysuccinimide and N,N dicyclohexyl carbodiimide.The synthesis product was characterized by the traditional sodium lauryl sulfate-polyacrylamide gel electrophoresis£¨SDS-PAGE£© and electrospray ionization mass spectrometric£¨ESI MS£© method.The result showed that the ceftiofur artificial bovine serum albumin antigen was succesfully prepared and the binary complex ratio of ceftiofur to BSA was about 8.Herein,atomic force microscope£¨AFM£© was firstly used to characterize the ceftiofur artificial antigen.