Determination of Antioxidant D in Pressure-sensitive Adhesive and Its Products by High Performance Liquid Chromatography

KeyWord:antioxidant D  pressure-sensitive adhesive  high performance liquid chromatography

Abstract:

      A high performance liquid chromatographic(HPLC) method was developed for the determination of antioxidant D in pressure sensitive adhesive and its products£®The sample was dissolved with ethyl acetate£¬then precipitated by methanol.The extract was centrifugally separated for 5 min at a speed of 3 000 r/min£®After filtrated with filter membrane£¬the supernatant was separated on a C18 column by isocratic elution using acetonitrile-water as mobile phase at a flow rate of 0.5 mL/min£¬and determined by HPLC with diode-array detector(DAD)at 310 nm£¬quantified by the external standard method£®The conditions of sample pretreatment and chromatographic separation were optimized£¬and the confirmatory method of mass spectrometry used to quantitatively determine antioxidant D in the sample was established£®Under the optimal conditions£¬the method showed good linearities between peak areas and the contents in the ranges of 0.01-1 mg/L and 1-100 mg/L for antioxidant D£¬with a limit of quantitation of 1 mg/kg£®The established method was applied in the determination of antioxidant D in emulsion-type pressure-sensitive adhesive£¬hotmelt-type pressure-sensitive adhesive and solvent-type pressure-sensitive adhesive£¬and the spiked recoveries ranged from 96% to 100% with relative standard deviations(RSDs) of 1.1%- 3.1%£®The results indicated that this method was reliable£¬sensitive and repeatable£¬and could meet the requirement for analysis of antioxidant D in the pressure sensitive adhesive and its products.