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Determination of Squalene in Rices from Different Areas by Gas Chromatography-Tandem Mass Spectrometry |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:gas chromatography-tandem mass spectrometry(GC-MS/MS) squalene rice Soxhlet extract method |
Author | Institution |
李红,田福林,刘成雁,程光荣 |
辽宁省分析科学研究院辽宁省标准化体系建设工程技术研究中心 |
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Abstract: |
Different extraction methods of bioactive component squalene in rices were studied and a new method was developed for the determination of squalene in rices.The squalene in rices from different areas were extracted by Soxhlet extract method,and analyzed by gas chromatography-tandem mass spectrometry(GC-MS/MS).The results showed that the calibration curve was linear over the concentration of squalene in the range of 1-50 mg/L with a correlation coefficient of 0.998 7.The detection limit(LOD) was 0.1 mg/kg.The recoveries of squalene at two spiked levels of 5 mg/kg and 20 mg/kg were in the range of 96%-104% with relative standard deviations(RSDs) no more than 3.6%.The method was sensitive and reproducible,and could be used to distinguish effectively rices with different species,origins and qualities. |
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