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Investigation on Interaction of Bovine Serum Albumin with ZnS Nanoparticles by Fluorescence Spectroscopy |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:Nano-ZnS bovine serum albumin(BSA) fluorescence spectroscopy interaction static quenching |
Author | Institution |
陈稚,吴都督,张爽,林晓,龙烁杭,施志海,吴锐彬 |
1.广东医学院分析中心;2.广东医学院药学院;3.广东医学院医学检验学院 |
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Abstract: |
ZnS nanoparticles was synthesized using zinc acetate and sodium sulfide as starting materials in water solution.The morphology and crystal phase of nano-ZnS were characterized by transmission electron microscopy(TEM) and powder X-ray diffraction(XRD).The interaction of nanoparticles and bovine serum albumin(BSA) was investigated by fluorescence spectroscopy in pH 7.0 Tris-HCl buffer.The result showed that the interaction between BSA and ZnS nanoparticles could resulted in the endogenous fluorescence quenching of BSA,which belonged to a static quenching mechanism.The quenching rate constant was 1.88×1014 L?mol-1?s-1,the binding constant(Ka) and binding site(n) were 1.73×105 L?mol-1 and 0.83,respectively. |
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