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Preparation and Evaluation of α-Alkenyl-β-cyclodextrin Monolithic Column for Capillary Electrochromatography |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:monolithic column capillary electrochromatography(CEC) allyl glycidyl ether β-CD DL-amino acid enantiomer separation |
Author | Institution |
李英杰,丁爽,吕仁江,丁会敏,邓磊,侯学功 |
齐齐哈尔大学化学与化学工程学院 |
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Abstract: |
In alkaline conditions,4-( 3-acryloxy-2-hydroxy ) propyloxy-β-cyclodextrin( PCD ) was synthesized based on the reaction of 1-alklyloxy-2,3-epoxy propane(also named as allyl glycidyl ether,AGE) and β-cyclodextrin(β-CD).A new type β-cyclodextrin polymer chiral monolithic column for CEC was prepared by using the in-situ polymerization of PCD and GMA as functional monomers in the capillary tube.The prepared monolithic column was evaluated by separating racemic mixtures of three amino acids and a chial drug(guguaifenesin) in capillary electrochromatography mode.The influences of organic modifier,buffer pH value and temperature on separation were investigated.Under the optimal CEC conditions,all of enantiomers were baseline separated in 8 min.Results showed that the novel PCD monolithic column had good chiral recognition ability,better reproducibility and easy operation. |
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