A high performance liquid chromatographic method was established for the simultaneous determination of 5 classes of antibiotics such as chloramphenicols,nitromidazoles,quinolones,sulfonamides and tetracyclines including 15 antibiotics residues in honey.The honey sample was dissolved in phosphoric acid solution at pH 2.0 and then purified and enriched directly with PCX solid phase extraction small column.The target compounds were separated on HPLC using acetonitrile-phosphoric acid solution(pH 2.5) as mobile phase by gradient elution,and detected with ultraviolet detector at 274 nm and 315 nm.The calibration curves of 15 antibiotics(chloramphenicol,metronidazole,ornidazole,oxytetracycline,chlorotetracycline,doxycycline,sulfapyridine,ciprofloxacin,lomefloxacin,ofloxacin,gatifloxacin,sparfloxacin,sulfadiazine,sulfamerazine,sulfamethoxazole) were linear in the range of 0.2-20.0 mg/L.The average spiked recoveries ranged from 60% to 105% with relative standard deviations of 1.3%-6.9%.The limits of detection of this method were in the range of 9.70-12.54 μg/kg and the limits of quantitation were 32.34-41.80 μg/kg.The method showed the advantages of simple sample preparation,good selectivity and high sensitivity,and was suitable for the simultaneous determination of antibiotics in honey. |