Determination of 17 Phthalate Esters in Food by QuEChERS£¯High Performance Liquid Chromatography

KeyWord:phthalate esters£¨PAEs£©  QuEChERS  high performance liquid chromatography£¨HPLC£©  food

Abstract:

      A QuEChERS with high performance liquid chromatographic£¨HPLC£© method was developed for the determination of 17 phthalate esters£¨PAEs£©£¬e.g.DMEP£¬DMP£¬DEEP£¬DEP£¬DPhP£¬BBP£¬DIBP£¬DBP£¬DBEP£¬DPP£¬DCHP£¬BMPP£¬DHXP£¬DEHP£¬DNOP£¬DINP and DNP£¬in food samples£®The targeted compounds were extracted with acetonitrile£¬and purified by PSA-based QuEChERS£¨50 mg PSA and 150 mg MgSO4£©.The extract were separated on a C18 column with mobile phase composed of water and acetonitrile by gradient elution at a flow rate of 1.0 mL/min.The UV detection wavelength was set at 224 nm.Under the optimal conditions£¬the calibration curves of phthalate esters were linear in the ranges of 0.5-10 mg/L for DINP and 0.05-10 mg/L for the others with correlation coefficients£¨r2£© more than 0.99.The average recoveries of 17 analytes from 5 kinds of food matrixes were in the range of 70%-120% with RSDs£¨n=6£©less than 15%£®The detection limits£¨LODs£¬S/N=3£© of phthalate esters were 0.001-0.02 mg/L and the quantitation limits£¨LOQs£¬S/N=10£© were 0.05-0.5 mg/kg£®The proposed procedure was applied in the analysis of 432 real samples from Xiamen£¬Fujian province£¨China£©£¬in which 18 positive samples were found.The proposed method was simple and accurate£¬and could be applicable for the routine analysis of monitoring PAEs residues in foodstuffs.