Determination of 14 Heterocyclic Aromatic Amines in Meat Products by Liquid Chromatography-Ion Trap-Time of Flight Tandem Mass Spectrometry

KeyWord:liquid chromatography-ion trap-time of flight tandem mass spectrometry£¨LCMS-IT-TOF£©  meat products  heterocyclic aromatic amines£¨HAAs£©

Abstract:

      A rapid analytical method was developed for the determination of 14 heterocyclic aromatic amines£¨HAAs£© in meat products by liquid chromatography-ion trap-time of flight tandem mass spectrometry£¨LCMS-IT-TOF£©.The samples were extracted with acetonitrile.The extract was cleaned up with Phenomenex strata-X-C,eluted with methanol-ammonia£¨9¡Ã1£©,then evaporated with a stream of nitrogen to near dryness and redissoved with acetonitrile-water£¨1¡Ã9£©.The separation of HAAs was performed on a Phenomenex Kinetex 2.6¦Ì C18 100A £¨2.1 mm i.d.¡Á100 mm,2.6 ¦Ìm£©,using 30 mmol/L ammonium formate-acetonitrile as mobile phase by gradient elution.The analytes were detected under positive ion electrospray ionization mode.Results showed that the linear ranges of 14 HAAs were 5-250 ¦Ìg/L with limits of detection£¨S/N=3£© of 0.59-3.27 ¦Ìg/kg£®The mean recoveries of all the compounds at three spiked levels of 10,50,250 ¦Ìg/kg were in the range of 69%-107%,67%-106% and 76%-113%,respectively,with the corresponding RSDs£¨n=6£© of 1.5%-15.6%,1.3%-11.8% and 1.8%-9.7%,respectively.The established method showed a wide liner range and a high sensitivity,and could meet the requirements for the simultanous analysis of the HAAs in meat products.