Determination of Celastrol by Lanthanide Sensitized Fluorescence Quenching on Terbium- Enoxacin-SDS

KeyWord:celastrol  terbium  enoxacin  SDS  fluorescence quenching

Abstract:

      In NH3-NH4Cl buffer solution(pH 9.3),terbium(¢ó) reacted with enoxacin (ENX) to form fluorescence coordinate compound with the maximum excitation wavelength of 330 nm and the maximum emission wavelength of 545 nm.The presence of SDS obviously strengthened the fluorescence intensity,but the fluorescence intensity of the complex was diminished by continuously adding celastrol,while the locations of the excitation and emission wavelengths were kept the same.Based on this,a new fluorescence method was proposed for the determination of celastrol.The method is simple,rapid and sensitive.The linear range of celastrol is 5.2¡Á10-6-8.4¡Á10-5 mol/L and the detection limit is 4.1¡Á10-8 mol/L.