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| Simultaneous Separation and Analysis of 3 Flavonoid Components in Houttuynia cordata Thunb by Column Switching Technology |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:Houttuynia cordata Thunb flavonoids column switching HPLC |
| Author | Institution |
| 彭丽华，卢红梅，郭方遒，蔡品品，梁逸曾 |
中南大学化学化工学院中药现代化研究中心 |
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| Abstract: |
| A method was established for the separation of complex and low content components in Houttuynia cordata Thunb by column-switching technology employing two reverse phase liquid chromatographic columns.A Synergi Fusion RP column and an Acclaim-120 C18 column with 250 mm×4.6 mm i.d.were adopted as the precolumn and analytical column，respectively.The precolumn was eluted with methanol -0.05% phosphoric acid as mobile phase.The analytical column was eluted with 0.05% phosphoric acid-acetonitrile.Three flavonoid components in Houttuynia cordata Thunb were simultaneously separated and detected.The calibration curves were linear in the concentration range of 10.0-1 000.0 mg?L-1，with correlation coefficient not less than 0.998.The recoveries of three compounds in Houttuynia cordata Thunb ranged from 95% to 99% with RSDs less than 3%.The results showed that the developed method was simple and rapid，and could be used in the analysis of flavonoid components. |
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