Determination of T-2,Zearalenone and Deoxynivalenol in Wheat Using Liquid Chromatography-Tandem Mass Spectrometry

KeyWord:solid phase extraction£¨SPE£©  liquid chromatography-tandem mass spectrometry £¨LC-MS/MS£©  mycotoxins  wheat

Abstract:

      A liquid chromatography tandem mass spectrometric£¨LC-MS/MS£©method was developed for the simultaneous determination of T-2£¬zearalenone£¨ZEN£© and deoxynivalenol£¨DON£© in wheat.The sample was extracted with acetonitrile-water£¨80¡Ã20£©.The extract was purified with an Amino column£¨AccuBond-AMINO£¬500 mg£¬6 mL£©.Three myctoxins were completely separated on a ZORBAX Extend-C18 column£¨150 mm¡Á2.1 mm,1.8 ¦Ìm£©by gradient elution with methanol and 5 mmol/L ammonium acetate as mobile phase£¬and detected by positive ion electrospray ionization mass spectrometry£¨ESI MS£© under multiple reaction monitoring£¨MRM£© mode.The results showed that the linearities of T-2,ZEN and DON were in the concentration ranges of 0.5-500£¬5-500,10-2 000 ¦Ìg/L£¬respectively£¬with correlation coefficients of 0.998 9£¬0.999 7 and 0.999 1£¬respectively.The spiked recoveries of T-2£¬ZEN and DON at three spiked levels in wheat were in the ranges of 86%-94%£¬80%-101% and 81%-105%£¬with relative standard deviations£¨RSDs£¬n=5£© of 2.6%-5.5%£¬3.6%-8.9% and 2.2%-8.1%£¬respectively.The limits of detection for T-2£¬ZEN and DON were 0.5£¬8.0,10.0 ¦Ìg/kg£¬respectively.The method was low cost£¬sensitive and accurate£¬and was suitable for the simultaneous determination of T-2,ZEN and DON in wheat.