Determination of Platinum in Serum Samples by Inductively Coupled Plasma Mass Spectrometry with Microscale Flow Injection Based on a Heated Spray Chamber

KeyWord:heated spray chamber  microscale flow injection  inductively coupled plasma mass spectrometry  serum  platinum

Abstract:

      A heated single pass spray chamber-based microscale flow injection sampling system was developed for the analysis of platinum in serum samples by inductively coupled plasma mass spectrometry.It consisted of a homemade demountable capillary microflow nebulizer£¬a single pass spray chamber heated by means of resistive heating£¬an eight channel sixteen port multifunctional injector valve and a syringe pump equipped with a 10 mL syringe as well as a peristaltic pump.Effects of the dimensions of spray chamber£¬the heating temperature and the sampling loop volume on the intensity were investigated.The maximum intensity were obtained with the single pass spray chamber with an inner diameter of 9 mm and a length of 6 cm for the heated section.When it was heated up to 90 ¡æ and the sampling loop volume was selected to be 5 ¦ÌL£¬the intensity of 195Pt was enhanced by a factor of 2.31 and the precision of transient signals for 195Pt was reduced from 5.1% to 2.2%,a signal peak with good configuration was obtained.Compared with the conventional sampling system£¬the heated single pass spray chamber-based microscale flow injection sampling system offers a little better sensitivity and detection limit in the case of lower sample consumption.The relative standard deviations of peak heights of 10 replicate trials of 10 ¦Ìg/L standard solution and a serum sample were 2.9% and 3.3%£¬respectively.The contents of platinum in serum samples by the proposed method agreed well with those obtained by the conventional sampling system.The heated single pass spray chamber-based microscale flow injection sampling system can be applied in many fields£¬especially in the case of limited or rare samples.