Preparation and Certification of A Certified Reference Material Allure Red Solution and Its Uncertainty Evaluation

KeyWord:allure red  solution certified reference material  high performance liquid chromatography  uncertainty evaluation

Abstract:

      A methodology regarding the preparation and certification of certified reference material (CRM) of allure red solution was presented.After the qualitative analysis on pure substance of allure red by liquid chromatography-mass spectrometry(LC-LTQ/MS) and 1H NMR spectrum,the purity of allure red was certified by high performance liquid chromatography(HPLC),the purity certification of allure red was performed at 240 nm with methanol and 0.1 mol/L ammonium acetate solution as mobile phase,and the Intersil ODS-C18 column(250 mm¡Á4.6 mm,5 ¦Ìm) was used for analysis.The certification purity of allure red was 99.61%.To verify the result£¬six certification labs were performed,at the same time,the water content and volatile impurity were determined by the total gravimetric analysis(TGA)£®Allure red solution CRM was prepared by the gravity-volume method£®After the tests of homogeneity and stability£¬the concentration of allure red solution CRM was certified and the uncertainty was evaluated£®The certified result was 100 mg/L and the expanded relative uncertainty was 1.0%(k=2).The allure red solution CRM was approved as a national CRM and could be applied in food safety detection.