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Separation of Three Single Phosporic Acidic Adenosines by a Nonaqueous Capillary Electrophoresis Method and Its Application |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:nonaqueous capillary electrophoresis(NACE) single phosporic acidic adenosine nucleoside separation |
Author | Institution |
宋娟梅,成光,边志明,贾鹏 |
1.淄博市环境监测站;2.淄博建筑工程学校;3.郑州大学化学系 |
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Abstract: |
The separation of 2′-AMP,3′-AMP and 5′-AMP were investigated by a nonaqueous capillary electrophoresis(NACE) method.Effects of pH* value,nonaqueous solvent,different category buffer on the separation efficiency were studied.The optimum separation conditions are as follows:running medium:200 mmol?L-1 Tris-H3BO3 containing 50% acetonitrile,pH* value:10.0; applied electric field:25 kV; column temperature:25 ℃; detection wavelength:260 nm,and samples were injected from the cathodal end of the capillary by 50 mbar for 5 s.Under the optimal conditions,there are excellent linearities between peak heights and concentrations of the analytes in the concentration range of 1-100 mg/L.The spiked recoveries ranged from 88% to 106% with RSDs less than 4%.The method was applied in the determination of adenosine samples with satisfactory results. |
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