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Determination of L-Carnitine in Infant Formula Milk Powder by High-performance Liquid Chromatography-Mass Spectrometry |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:L-carnitine HPLC-MS infant formula milk powder |
Author | Institution |
竺琴,苏流坤,郑家概,农云军 |
中国广州分析测试中心广州化学危险应急检测技术重点实验室 |
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Abstract: |
A high-performance liquid chromatography-mass spectrometric method was developed for the determination of L-carnitine in infant formula milk powder.After pretreatment procedure,the sample was separated on a C4 (4.6 mm×250 mm,5 μm) column using methanol-water (10∶90) as mobile phase with a flow rate of 1.0 mL?min-1,a column temperature of 25 ℃ and injection volume of 10 μL.The split ratio of post column was 1∶3.The target compound was detected under multiple reaction monitoring(MRM) mode with positive ion using m/z 162.2→103.1 as the quantitation ions.Each analysis was completed within 10 min.A good linear calibration for L-carnitine was obtained in the range of 0.40-10.0 mg?L-1 with a correlation coefficient of 0.999 3.The spiked recoveries were in the range of 87%-106%.The limit of detection(S/N=3) and limit of quantitation(S/N=10) of L-carnitine were 39.0 ng and 116.9 ng,respectively.The proposed method was simple,sensitive and reproducible,and was suitable for the determination of L-carnitine in infant formula food. |
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