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| Determination of Methyl Selenocysteine in Middle-aged Milk Powder by Gas Chromatography-Triple Quadrupole Mass Spectrometry |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:methyl selenocysteine gas chromatography-triple quadrupole mass spectrometry middle-aged milk powder ethyl chloroformate |
| Author | Institution |
| 廖且根，李伟红，罗林广 |
江西省农业科学院农产品质量安全与标准研究所，江西省农业科学院农产品质量安全重点实验室 |
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| Abstract: |
| A gas chromatography-triple quadrupole mass spectrometric method was developed and validated for the determination of methyl selenocysteine in middle-aged milk powder.The analytes were extracted with trichloroacetic acid and acetonitrile,and derived with ethyl chloroformate in ethanol-pyridine.Methyl selenocysteine was separated on an HP-5MS micro-column,and quantified under multiple reaction monitoring mode.The calibration curve was linear in the range of 0.005 – 1.0 mg/L,with detection limit of 0.01 mg/kg and quantitation limit of 0.05 mg/kg.The average recoveries of methyl selenocysteine from middle-aged milk matrixes at the spiked level of 0.05,0.2,0.4 mg/kg ranged from 79% to 86% with relative standard deviations of 2.8%-4.0%.The method could meet the requirement for determination of methyl selenocysteine in middle-aged milk powder. |
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