Determination of Aquaculture Disinfectants Residues in Aquatic Products by Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry

KeyWord:hydrophilic interaction  liquid chromatography-tandem mass spectrometry£¨LC-MS/MS£©  aquaculture disinfectants  triclosan  dichloroisocyanurate  trichloroisocyanurate  aquatic products

Abstract:

      A new method for the determination of 3 aquaculture disinfectants in aquatic products using hydrophilic interaction liquid chromatography-tandem mass spectrometry£¨LC-MS/MS£© was established.The homogenized sample was extracted with acetonitrile-5% ammonia hydroxide£¨30¡Ã70£¬by volume£©.Separation of analytes was performed using a SeQuant ZIC-HILIC hydrophilic interaction column by gradient elution after solid-phase extraction£¨SPE£© purification.The qualitative and quantitative analyses of 3 aquaculture disinfectants were operated by electrospray ionization mass spectrometry under the negative mode using multiple reaction monitoring£¨MRM£©.Acetonitrile and water with 0.1% formic acid and 10 mmol/L ammonium acetate were used as the mobile phase.The calibration curves were linear in the range of 0.042-2.4 mg/L with correlation coefficients more than 0.99.The average recoveries at three spiked concentration levels varied from 68% to 96% with relative standard deviations£¨RSDs£© of 5.9%-10.9%.The limit of detection£¨LOD£¬S/N¡Ý3£© and limit of quantitation£¨LOQ£¬S/N¡Ý10£© were 0.015 mg/kg and 0.05 mg/kg£¬respectively.The real sample tests showed that the method was suitable for the determination of aquaculture disinfectants in aquatic products.