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Using Lipase Modified Screen printed Electrode as Glyceryltrioleate Biosensor |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:Screen-printed electrode lipase glyceryltrioleate |
Author | Institution |
刘韩凤,郭春景,叶永康,罗水忠,姜绍通 |
合肥工业大学生物与食品工程学院 |
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Abstract: |
A rapid method for the determination of glyceryltrioleate was developed by using screen-printed electrode(SPE) modified with lipase as an electrochemical biosensor.Cyclic voltammetry(CV) and chronocoulometry(CC) were applied to characterize the behavior of glyceryltrioleate on the surface of the lipase-modified SPE.The optimal conditions of glyceryltrioleate determination were investigated.A linear response to glyceryltrioleate in the concentration ranges of 001-5 g?L-1 with a detection limit of 2.5 mg?L-1 for CV,and 0.001-5.50 g?L-1 with a detection limit of 0.2 mg?L-1 for CC were obtained.The method was used in the determination of 6 sunflower seed oil samples with spiked recoveries of 94%-105%. |
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