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Rapid Determination of Benzo[a]pyrene in Edible Vegetable Oil Using a New Type of Solid Phase Extraction Column |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:Bond Elut ENV solid phase extraction column edible vegetable oil benzo[a]pyrene silica gel solid phase extraction column liquid chromatography with fluorescence detection |
Author | Institution |
何强,孔祥虹,李建华,赵洁,张莹 |
1.陕西出入境检验检疫局检验检疫技术中心;2.西安理工大学理学院 |
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Abstract: |
A new type of solid phase extraction column(Bond Elut ENV)was used for the rapid determination of benzo[a]pyrene in edible vegetable oil.And a solid phase extraction purification and high performance liquid chromatography/fluorescence detection method was developed for the determination of benzo[a]pyrene in edible vegetable oil.Samples were dissolved in n-hexane and then purified by solid phase extraction.Chromatographic analysis was performed on a SUPELCOSILTM LC-PAH(25 cm×4.6 mm,5 μm) column using acetonitrile-water (95∶5) as mobile phase.Quantitation analysis was carried out by the external standard method using fluorescence detecter at λex=297 nm and λem=408 nm.The calibration curve was linear over the benzo[a]pyrene concentration in the range of 1.0-50.0 μg/L(r=0.999 6) with detection limit of 0.3 μg/kg.The recoveries ranged from 79% to 102% with relative standard deviations no more than 94%.The method is accurate,practical,simple and fast,and has an extensive application prospect in the determination of benzo[a]pyrene residues in edible vegetable oil. |
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