Simultaneous Separation and Determination of Three Active Ingredients in Paracetamol Caffeine Aspirin Powder by Micellar Electrokinetic Capillary Chromatography

KeyWord:micellar electrokinetic chromatography  Paracetamol Caffeine Aspirin Powder  caffeine  paracetamolum  aspirin

Abstract:

      A micellar electrokinetic capillary chromatographic£¨MECC£© method was established for the separation and determination of three active components£¬including caffeine£¬paracetamolum and aspirin£¬in Paracetamol Caffeine Aspirin Powder.The micellar phase of cholic acid combined with improver of organic base triethanolamine was optimized.The optimal electrophoretic conditions were as follows£ºbuffer£º40 mmol/L borate-60 mmol/L cholic acid-12.5% triethanolamine £¨pH 12.0£©£¬separated voltage£º15 kV,detection wavelength£º254 nm.Under the optimum conditions£¬the baseline separation of three components in Paracetamol Caffeine Aspirin Powder was achieved within 14 min.The linear calibration curves were in the ranges of 18.75-900.0 mg/L £¨r=0.999 9£© for caffeine£¬2.60-62.50 mg/L £¨r=0.992 9£© for paracetamolum and 79.17-3 800 mg/L £¨r=0.994 0£© for aspirin.The relative standard deviations of three components were no more than 1.8% for migration time and 6.8% for peak height.The detection limits £¨S/N=3£© were 4.6£¬0.17,38 mg/L£¬respectively.The spiked recoveries of three components in Paracetamol Caffeine Aspirin Powder were 100%£¬100% and 102%£¬respectively£¬which could meet the requirement for analyzing reality sample.The improved method is simple£¬reliable£¬sensitive and economical£¬and is suitable for the separation and determination of the multiple ingredients in Paracetamol Caffeine Aspirin Powder.