Determination of Trace Ephedrine and Pseudoephedrine in Urine Sample by Hollow Fiber-Liquid Phase Microextraction and HPLC

KeyWord:HF-LPME  HPLC  ephedrine  pseudoephedrine  urine sample

Abstract:

      A simple and sensitive method based on hollow fiber liquid phase microextraction£¨HF-LPME£© was developed to separate£¬ purify and enrich ephedrine and pseudoephedrine in human urine sample.With HF-LPME device£¬the extraction conditions were optimized.Under the optimal conditions using n-octyl alcohol as organic phase impregnated in the pores of hollow fiber£¬and acidic microliter solution£¨pH 2.0£©as acceptor phase£¬the enrichment factors of 180 for ephedrine and 220 for pseudoephedrine were obtained at room temperature with extraction time of 60 min.The calibration curves- were linear in the ranges of 0.01-5 mg/L and 0.005-0.75 mg/L for ephedrine and pseudoephedrine,with their correlation coefficients of 0.998 2 and 0.997 8£¬respectively.The limits of quantitation£¨S/N=10£© were 0.01 mg/L and 0.005 mg/L£¬respectively.With the advantages of low organic solvent consumption and high extraction efficiency£¬ the method was suitable for the simultaneous determination of ephedrine and pseudoephedrine in urine sample.