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Determination of Maleic Acid and Maleic Anhydride in Starch Products by High Performance Liquid Chromatography |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:maleic acid maleic anhydride high performance liquid chromatography(HPLC) starch products |
Author | Institution |
李红艳,陈万勤,王瑾,张水锋,沈潇冰,邵亮亮,刘柱,陈小珍 |
浙江省质量技术监督检测研究院 |
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Abstract: |
A high performance liquid chromatographic(HPLC) method was established for the analysis of the total contents of maleic acid and maleic anhydride in starch products.A better extraction method was developed by using 5% ethanol as extraction solvent and ultrasonic extraction time of 10 min.The optimal chromatographic separation conditions were as the follows:separation column:Plastisil ODS C18 column(250 mm×4.6 mm,5 μm);mobile phase:methanol-1‰ phosphoric acid(2∶98);detection wavelength:214 nm;flow rate:1.0 mL/min;column temperature:30 ℃.The limit of quantitation for formaleic acid was 5.0 mg/kg,and the linear range were in the range of 0.25-100 mg/L with a correlation coefficient of 0.999 7.The average spiked recoveries were between 88% and 89% with RSDs(n=5) less than 2%.The method could satisfy the requirement for the determination of the total contents of maleic acid and maleic anhydride in actual sample. |
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