| Erythropoietin（EPO） is one kind of important hematopoietic growth factor,which is sometimes abused by endurance athletes to enhance performance since it can improve the oxygen-carrying capacity of blood.Benefited from the specific recognition and the amplification capability of aptamer,an analysis strategy for the quantification of recombinant human erythropoietin-α（rHuEPO-α） by magnetic separation-based aptameric real-time quantitative PCR was established.As for the serious interference effects caused by the high abundant proteins in the real biological samples such as urine and plasma et al,according to the base pairing principle,two complementary strands which could respectively bind primer regions at both ends of aptamer in this study were designed and synthesized,followed by evaluation by electrophoretic mobility shift assay（EMSA）,and the optimal sequence was linked to streptavdin-paramagnetic particles（SA-PMPs） via the biotin-streptavidin interaction,thus the formed probes could efficiently capture the aptamer-target complexes from complicated matrix.The results showed that the established analysis strategy could be successfully applied in the determination of the concentration of rHuEPO-α in human serum with this sample pretreatment,with a limit of detection（LOD） of 25 pmol/L and a linear range from 50 pmol/L to 50 nmol/L. |