Rapid Determination of 29 Pesticide Residues in Cereals and Oil Crops by Online Gel Permeation Chromatography Coupled with Gas Chromatography-Mass Spectrometry

KeyWord:online gel permeation chromatography coupled with gas chromatography-mass spectrometry(GPC/GC-MS)  pesticide residues  cereals  oil crops

Abstract:

      A based on automated online gel permeation chromatography coupled with gas chromatography-mass spectrometry(GPC-GC/MS) method was developed for the rapid determination of 29 pesticide residues in cereals and oil crops.Pesticides were extracted from cereals and oil crops samples with acetonitrile and decontaminated via primary secondary amine(PSA) sorbents,then determined by GPC/GC-MS.The identification and detection of target compounds were achieved in electron impact(EI) mode under selected ion monitoring(SIM) mode.The calibration curves of 29 pesticide residues were linear in the range of 0.25-600 ¦Ìg?L-1 with good correlation coefficients more than 0.99.The recoveries for all compounds in feeds at three fortified levels of 1MRL,2MRL and 4MRL were in the range of 65%-148%.The limits of quantitation(LOQs,S/N=10) for 11 pesticides(e.g.dieldrin,parathion-ethyl and methamidophos,etc.) were in the range of 2.0-6.0 ¦Ìg?kg-1 and those of the other 18 pesticides(such as dichlorvos,dimethoate and HCH,etc.) were less than 1.0 ¦Ìg?kg-1.The method was simple,rapid and reliable,and could meet the require-ment for the simultaneous determination and analysis of 29 pesticide residues in cereals and oil crops.