Fast Determination of Three Acid Orange Dyes£ºAcid Orange ¢ò£¬Acid Orange ¢ó and Acid Orange AGT in Meat Products by Ion-pair Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

KeyWord:ultra performance liquid chromatography tandem mass spectrometry  multi reaction monitoring  acid orange ¢ò  acid orange ¢ó  acid orange AGT  ion-pair  meat product  food safety

Abstract:

      A method based on ultra performance liquid chromatography-tandem mass spectrometry£¨UPLC-MS/MS£© was developed for the simultaneous determination of three industrial acid dyes£¬namely acid orange ¢ò£¬acid orange ¢ó and acid orange AGT£¬in meat products.The samples were extracted with acetonitrile£¨containing 5% ammonia£©£¬cleaned up and enriched with WAX solid phase extraction£¨SPE) cartridge£¬and then analyzed by UPLC-MS/MS under the multiple reaction monitoring£¨MRM£© mode.All the analytes were successfully detected in negative ion mode by adding an ion-pair reagent of dihexylammonium acetate£¨DHAA£© to the mobile phase in 6 min.The calibration curves were linear between 5.0 ¦Ìg/L and 300 ¦Ìg/L£¬and the correlation coefficients£¨r£© were more than 0.999.The limits of detection£¨LOD£¬S/N=3£© and the limits of quantitation£¨LOQ£¬S/N=10£© were in the range of 20.0-50.0 ¦Ìg/kg and 70.0-170.0 ¦Ìg/kg£¬respectively.The spiked recoveries were in the range of 83%-89%£¬and relative standard deviations £¨RSD£¬n=3£© were no more than 7.5%.The method was simple£¬effective and sensitive£¬and was suitable in the determination and confirmation of three industrial acid orange dyes in the meat product samples.