| Cyromazine molecularly imprinted polymers（MIPs） were synthesized by UV photo-initiation and bulk polymerization method at low temperature,using cyromazine as template molecule，methacrylic acid（MAA） as functional monomer, ethylene dimethacrylate（EDMA） as cross linker,toluene and isooctane as binary porogen.Comparative determination and characterization of the prepared MIP and NIP were carried out by equilibrium adsorption experiment,scanning electron microscope（SEM）,infrared absorption（IR） and mercury analyzer（MA）.The results showed that the MIPs possessed the specific adsorptivity toward the template molecule.Scatchard plot′s analysis revealed that the apparent maximum binding capacity and the dissociation contents were as the follows：Qmax1=119 μmol/g，Kd1=131 μmol/L；Qmax2=2.46 μmol/g，Kd2=4.84 μmol/L.It was demonstrated that the proposed MI-SPE/HPLC method could be successfully applied in the determination of cyromazine in cucumber sample.A linear calibration curve was obtained in the concentration range of 0.1-10 mg/L（r=0.999 1） with a detection limit of 0.05 mg/L.The established method was simple and rapid,and had a better effect than commercial SPE column. |