| A thin layer chromatography(TLC）-fluorescence scanning method was established for the determination of camptothecine(CPT） and 10-hydroxycamptothecine(10-HCPT） in Chinese herbal medicine Xishuguo(Common camptotheca fruit,CCF）.Methanol was used as solvent to extract CPT and 10-HCPT in CCF sample.The separation was carried out on silica gel H plate using chloroform-acetone(7∶3） as developing agent.The determination was accomplished by fluorescence linear scanning with excitation wavelength of 350 nm.In the quality range of 0.010 5-0.063 0 μg,there is a linear relationship between integral fluorescence intensity and quality of CPT.The regression equation was A=474.0+159 881.2 m(μg） with a correlation coefficient of 0.998.The spiked recovery of CPT was 99%.In the quality range of 0.003 3-0.033 0 μg,a linear relationship between integral fluorescence intensity and quality of 10-HCPT was observed,and the regression equation was A=51.3+59 486.2 m(μg） with a correlation coefficient 0.999.The spiked recovery of 10-HCPT was 96%.This method was simple and repeatable.Using this method,the contents of CPT and 10-HCPT in a CCF sample were determined to be 0.122% and 0.013%,respectively. |