Determination of Cefotaxime Residue in Milk by Molecular Imprinted Solid Phase Extraction/Capillary Electrophoresis

KeyWord:molecularly imprinted solid phase extraction  capillary electrophoresis  cefotaxime  milk

Abstract:

      A molecularly imprinted polymer for cefotaxime£¨CTX-MIP£© was synthesized by the molecular imprinting technique using cefotaxime£¨CTX£© as template molecule,¦Á-methacrylic acid£¨MAA£© as functional monomer,ethylene glycol dimethacrylate£¨EGDMA£© as cross linking agent.By using the cefotaxime molecularly imprinted polymer as sorbent in solid-phase extraction column,a molecularly imprinted solid phase extraction-capillary electrophoresis£¨MISPE-HPCE£© method was developed for the determination of cefotaxime residue in milk.The results showed that cefotaxime imprinted polymer exhibited a higher selectivity to cefotaxime compared with that of the non imprinted polymer.The linear range of cefotaxime was in the range of 0.5-100 mg/L£¬and the correlation coefficient was 0.999 4.The recoveries at three spiked concentration levels of 10,50,100 mg/L were 78%,84% and 86%,respectively,with RSDs of 3.5%-4.5%.The limit of detection(LOD,S/N¡Ý3£© and the limit of quantitation(LOQ,S/N¡Ý10£© were 98.52 ¦Ìg/L and 329.0 ¦Ìg/L,respectively.The method could meet the requirements for veterinary drug residue analysis.