Fingerprint Analysis of Deng-s Herbal Tea Granule by Ultra High Performance Liquid Chromatography Coupled with Mass Spectrometry

KeyWord:ultra high performance liquid chromatography-mass spectrometry  fingerprint  simi-larity evaluation  princIpal component analysis  Deng-s herbal tea granule  quality control

Abstract:

      A fingerprint analysis method was developed for the quality control of Deng-s herbal tea granule(DHTG) by ultra high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS).The samples were extracted by Soxhlet extraction with methanol as solvent for 60 min.UHPLC-MS separation was performed on a Waters ACQUITY HSS T3 C18(150 mm¡Á3.0 mm,1.8 ¦Ìm) column by gradient elution with 0.5% formic acid-acetonitrile as mobile phase,at the temperature of 35 ¡æ and a flow rate of 0.8 mL/min.The base peak chromatograms(BPC) obtained with negative-ion electrospray ionization(ESI) mass spectra were selected for the fingerprint analysis.A good separation of 32 common peaks was achieved in 15 min,and 15 of them were confirmed by reference substances.Similarity evaluation was performed by a professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004A),and the similarity among 15 batches of samples was no less than 0.960,which confirmed that the quality of DHTG was stable.The principal component analysis(PCA) was performed by using the samples as variables and the relative peak areas of 32 common peaks as observations.A fine difference among DHTG samples was expressed in the PCA result.The developed method was fast and efficient,and was suitable for the quality control of DHTG.