| A new method was developed for the determination of carbofuran,carbaryl and atrazine in environmental water by ultrahigh-pressure liquid chromatography(UPLC) combined with solid phase extraction(SPE).Through the investigation of the effects of mobile phase,UV detection condition,SPE cartridges,SPE load flow rate and filter materials,the optimum conditions were obtained.Water sample was loaded on the Bond Elute Plexa SPE cartridges with a flow rate of 5-10 mL/min.The analytes were eluted with methylene chloride in SPE cartridges.The eluted solvent was concentrated and redissolved in methanol/water(1∶1).The analysis conditions were as the follows:UV wavelength of detection:222 nm,chromatographic column:ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7 μm),mobile phase:methanol-water(55∶45), flow rate:0.4 mL/min.Under the optimal conditions,three analytes were separated by baseline within 1.5 min.The correlation coefficients of carbofuran,carbaryl and atrazine standard curves were more than 0.999 in the range of 0.1-2.0 mg/L.The relative standard deviations of nine parallel injections of carbofuran,carbaryl and atrazine were 1.7%,0.2% and 0.7%,respectively,and their method detection limits(S/N=3) were 0.04,0.003,0.004 μg/L,respectively.The recoveries of three compounds at high and low spiked levels were in the range of 74%-94%. This method has the advantages of saving time,simple operation and sensitivity,and could be applied in the detection of trace carbofuran,carbaryl and atrazine in environmental water. |