Accurate Quantitative Analysis Method for 8 Kinds of Mycotoxins in Cereal

KeyWord:isotope dilution assay  cereal  mycotoxins  high performance liquid chromatography-tandem mass spectrometry

Abstract:

      A high performance liquid chromatography-tandem mass spectrometric£¨HPLC-MS/MS)method was developed for the determination of 8 kinds of mycotoxins£¨aflatoxin B1£¬aflatoxin B2,aflatoxin G1,aflatoxin G2£¬fumonisin B1£¬fumonisin B2£¬sterigmatocystin and roquefortine C) in cereal.The sample was diluted with hexane to remove the oil,then extracted with 60% acetonitrile.The acetonitrile layer was made up to constant volume and passed through the filter before tested.The detection was performed in the electrospray ion positive mode and multiple reaction monitoring£¨MRM) mode£¬and the quantitation of target compounds was carried out by the isotope dilution assays.The results indicated that the 8 compounds showed good linear relationships in certain concentration ranges,with correlation coefficients not less than than 0.997 0.The average recoveries ranged from 77% to 123%,with relative standard deviations of 0.6%-13.3%.The method was proved to be rapid and sensitive,and could be used for the detection of mycotoxins in cereal.