| A new method,termed as “pending templates imprinting”,to prepare protein-imprinted polymers was proposed.The molecular imprinted polymers were prepared in the presence of a real sample(chicken egg white),rather than any known purified proteins.An egg white protein-imprinted monolithic column and a control column were prepared by in situ polymerization.By comparing the electrophoretic separations of the sample in the imprinted polymeric and the control capillary,it is found that such a sample-imprinted polymer could retain abundant components of the original sample,the egg white.As a result,the signals of abundant proteins in the sample were removed or reduced in the electropherogram.Meanwhile,new peaks appeared,which stands for the enrichment of the other minor proteins in the same sample.This suggests a new way to investigate molecular imprinting,which means analyzing complicated samples based on their instinctive complexities,i.e.,through a series of polymers just imprinted by themselves. |