Determination of 16 Sulfonamide Residues in Animal-originated Foods by High Performance Liquid Chromatography with Immunoaffinity Column

KeyWord:sulfonamides(SAs)  immunoaffinity column  high performance liquid chromatography

Abstract:

      An immunoaffinity column purification and high performance liquid chromatographic£¨IAC/HPLC£© method was established for the simultaneous determination of 16 sulfonamide(SAs) residues in animal-originatal food products.The samples were extracted with 80% ethanol,cleaned-up with an immunoaffinity column,and analyzed by HPLC.The separation of 16 SAs was performed on an Agilent Eclipse XDB-C18 column using methanol(A)-pH 3.5 phosphate buffer(B) as mobile phase by gradient elution at a flow rate of 1.0 mL/min.The detection wavelength was set at 270 nm.The injection volume was 50 ¦ÌL.All of 16 SAs residues were simultaneously separated and determined by HPLC within 42 min.Linear ranges of 16 SAs were in the range of 10-200 ¦Ìg/L with correlation coefficients more than 0.999.The average recoveries at spiked concentration levels of 20,50 and 100 ¦Ìg/kg ranged from 62% to 104%,with relative standard deviations(RSDs) of 0.9%-6.6%.The detection limits(S/N=3) ranged from 4 ¦Ìg/kg to 10 ¦Ìg/kg.The method was suitable for the determination of sulfonamide residues in the edible animal products,with advantages of simplicity£¬rapidness£¬sensitivity and environment friendliness.