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Determination of Cartap Residue in Tea Using Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:hydrophilic interaction chromatography-tandem mass spectrometry(HILIC-MS/MS) cartap tea matrix effect |
Author | Institution |
陈红平,刘新,王川丕,汪庆华,蒋迎 |
中国农业科学院茶叶研究所农业部茶叶质量监督检验测试中心 |
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Abstract: |
A method combining hydrophilic interaction liquid chromatography(HILIC) with tandem mass spectrometry(MS/MS) was developed for the determination of cartap residue in tea.The target analyte was extracted with acetonitrile-methanol-acetate acid(93∶5∶2) and cleaned up using solid phase extraction(SPE) cartridge with C18 or dispersive solid phase extraction(d SPE) with GCB and C18.The analyte was separated on a ZIC HILIC silica column by a gradient elution of acetonitrile and 10 mmol/L ammonium acetate.A tandem mass spectrometry with electrospray ionization(ESI) was employed to detect cartap using multi-reactionmonitoring(MRM) mode.The matrix effect depended on tea categories and matrix content.A satisfactory linearity(r2≥0.998) was obtained in the concentration range of 0.005-0.5 mg/L.The limits of quantitation(LOQs) for green tea and black tea were 0.008 mg/kg and 0.005 mg/kg,respectively.The method was validated at the spiked levels of 1LOQ,5LOQ and 10LOQ in green tea and black tea,and the recoveries ranged from 70% to 89% with relative standard deviations(RSDs) less than 6%.The results demonstrated that the method was sensitive and accurate,and was suitable for the determination of cartap residue in tea. |
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