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Rapid Determination of Caffeine in Milk Tea by Pseudo Reversed Phase Liquid Chromatography |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:caffeine milk tea rapid determination pseudo reversed phase liquid chromatography |
Author | Institution |
董丽丽,于玲,李敏,王力川 |
邢台学院生物化学系 |
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Abstract: |
A rapid and sensitive method of pseudo reversed phase liquid chromatography(pseudo-RPLC) was developed for the analysis of caffeine in milk tea.The samples were separated on a bare silica column coupled with aqueous-rich mobile phase(pH 2.9,0.03 mol?L-1sodium formate-acetonitrile,95∶5),and tested within 5 min.The calibration curve was linear in the concentration range of 0.2-1 000 mg?L-1 with correlation coefficient of 0.999 5.The recoveries at a spiked level of 20 mg?L-1 ranged from 98% to 103% with RSDs 1.9%-3.6%. |
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