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Simultaneous Determination of Four Tocopherols in Vegetable Oilsby Reverse Phase High Performance Liquid Chromatography |
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DOI:10.3969/j.issn.1004-4957.年份.月份 |
KeyWord:reverse phase high performance liquid chromatography(RP-HPLC) fluorescence detection tocopherols vegetable oils |
Author | Institution |
于瑞祥,张欣,张秀芹,方晓明,丁卓平 |
1.上海海洋大学食品学院;2.上海出入境检验检疫局 |
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Abstract: |
A method for the simultaneous determination of four tocopherols in vegetable oils by reverse phase high performance liquid chromatography(RP-HPLC) was established.The sample was extracted with methanol under ultrasonic.The chromatographic separation was carried out on a C30 column(4.6 mm i.d.×250 mm,5 μm) using fluorescence detection at 290 nm of excitation wavelength and 340 nm of emission wavelength.Methanol-water-tert-butyl methyl ether(60∶15∶25)was used as mobile phase by isocratic elution.Results showed that good linear ranges for 4 tocopherols were obtained from 0.050 mg/L to 100.0 mg/L with correlation coefficients(r2)larger than 0.999.The mean recoveries were in the range of 81.3%-93.2% at three spiked levels of 0.50,50.0,500.0 mg/kg,and the relative standard deviations(RSDs) were less than 6%.The limits of detection(LODs) were 0.10 mg/kg for α-tocopherol,0.05 mg/kg for β- and γ-tocopherol,and 0.02 mg/kg for δ-tocopherol. The method is accurate,sensitive and reliable,and could be applied in the real sample analysis. |
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