Simultaneous Determination of 5 Preservatives Residues in Fresh Fruit by Gas Chromatography-Mass Spectrometry

KeyWord:gas chromatography-mass spectrometry£¨GC-MS£©  fresh fruit  preservatives  residue amount

Abstract:

      A gas chromatography- mass spectrometric£¨GC-MS£© method was established to simultaneously detect 5 kinds of fruit preservatives including p-methyl hydroxybenzoate£¬p-ethyl hydroxybenzoate£¬ethyl naphthol£¬4-phenyl phenol and diphenyl ether.The samples were extracted with ether£¬concentrated by nitrogen blowing£¬and purified with activated carbon column.The analysis of target compounds was performed by GC-MS under the selected ion mode£¨SIM£©,and quantified by the external standard method.Under the optimized conditions£¬the calibration curves of the 5 preservatives were linear in the range of 0.2-4.0 mg/L with correlation coefficients£¨r2£© more than 0.991.The spiked recoveries ranged from 80.4% to 104.5% with relative standard deviations £¨RSDs£© of 1.2%- 6.4%.The limit of detection of diphenyl ether was 0.05 mg/kg£¬and those of the other analytes were all 0.1 mg/kg.The method was simple£¬rapid and cheap£¬and the impurities like pigments in fruit samples could be diminished with activated carbon column.Therefore£¬the method was suitable for the determination of the 5 preservatives residues in fresh fruits with high sensitivity and selectivity.